In Vivo Antitumor Effect against Murine Cells of CT26 Colon Cancer and EL4 Lymphoma by Autologous Whole Tumor Dead Cells

Figure 6

ICBs-CT26 characterization. (a) Size and morphology of live CT26 cells and ICBs-CT26 were evaluated by confocal microscopy using CellTraker™ CMFDA Green and Hoechst 33342 shown at the top and bottom panels, respectively. (b) CT26 live cells and ICBs-CT26 area was measured, calculated, and expressed as . (c) The ability of the DCs to phagocytose ICBs-CT26 was evaluated using syngeneic BM-DCs cells (DCs) generated as described in methods. DCs were challenged with ICBs-CT26 stained with CellTraker™ CMTPX (pseudocolor green). Representative histograms show the CD11c+ population that acquired the CMTPX from the ICBs-CT26 (green peak). Negative control is showed with DCs alone (black peak) and DCs-ICBs-EL4 without staining (grey peak). Latex beads were used as positive control (blue peak). 7-AAD+ cells were excluded to avoid detection of ICBs-CT26 unspecific bound to DCs. The asterisks () represent statistically significant effects (). .

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