In Vivo Antitumor Effect against Murine Cells of CT26 Colon Cancer and EL4 Lymphoma by Autologous Whole Tumor Dead Cells

Figure 8

ICBs-CT26 lead maturation of syngeneic BM-DCs. The effect of ICBs-CT26 on levels of CD40 (a), CD86 (b), MHC-I (c), MHC-II (d) markers, and TNFα (e) and IL-12 (f) cytokine in syngeneic BM-DCs after 24 hrs of stimulation. BM-DCs challenged with CT26-ICBs correspond to green circles, untreated DCs correspond to black circles, and LPS treated correspond to open circles. Graphics show normalized median fluorescence intensity (MFI); this was calculated as a ratio between the MFI of the DCs treated with ICBs-CT26 and the MFI of the untreated DCs or DCs alone. TNFα (e) and IL-12 (f) cytokine productions are shown in representative histograms for untreated DCs (left panels), BM-DCs challenged with CT26-ICBs (middle panels), and with LPS (right panel). Representative histograms are shown in all cases. Grey lines represent to LPS, black line corresponds to untreated DCs as negative control, and green corresponds to the treatment with ICBs-CT26. Data corresponded to at least 5 independent experiments and were expressed as . Asterisks () represent statistically significant effects ().

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