[Retracted] Human Schlafen 5 Inhibits Proliferation and Promotes Apoptosis in Lung Adenocarcinoma via the PTEN/PI3K/AKT/mTOR Pathway

Figure 1

The effects of SLFN5 knockdown and overexpression on proliferation of A549 lung cancer cells. (a) Western blot analysis of SLFN5 protein expression in A549 lung cancer cells stably transfected with control shRNA (ShCtrl), SLFN5 shRNA (ShSLFN5), lentiviral vector (Vector), and lentiviral SLFN5 (SLFN5). (b) Immunofluorescence staining of SLFN5 (red) and DAPI (blue) in A549 cells stably transfected with ShCtrl or ShSLFN5. Scale bar, 50 μm. (c) Cell numbers in response to increasing or decreasing levels of SLFN5 (, ). (d) Proliferation by CCK-8 assay in response to increasing or decreasing levels of SLFN5 (, ). (e) Immunofluorescence staining of Ki-67 (red) and DAPI (blue). Percentages of Ki-67-positive cells (, ). Scale bar, 100 μm. (f) Colony formation results (, ). (g) Western blot analysis of cyclin D1 expression with densitometry graphs shown below.

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