[Retracted] Human Schlafen 5 Inhibits Proliferation and Promotes Apoptosis in Lung Adenocarcinoma via the PTEN/PI3K/AKT/mTOR Pathway

Figure 4

SLFN5 regulates p-AKT/GSK-3β/β-catenin and p-AKT/mTOR pathways via transcriptional regulation of PTEN mRNA. (a) Western blot analysis of protein expression of p-AKT, GSK-3β, β-catenin, mTOR, and PTEN in A549 cells stably transfected with ShCtrl, ShSLFN5, Vector, or SLFN5. (b) Real-time PCR analysis of PTEN in A549 cells stably transfected with ShCtrl, ShSLFN5, Vector, or SLFN5. (c) Immunohistochemistry and H&E staining of SLFN5 and PTEN in lung cancer samples. Red arrowheads indicate cancer cells, and black arrowheads indicate lymphocytes in the cancer stroma. Scale bars, 100 μM. Positive expression analyses of PTEN and SLFN5 were shown as

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