Electroacupuncture Inhibits Autophagy of Neuron Cells in Postherpetic Neuralgia by Increasing the Expression of miR-223-3p

Figure 1

Effect of EA on RTX-induced mechanical allodynia. . Time course of mechanical withdrawal threshold in response to von Frey filaments. Control: Sprague-Dawley rats; model: injected with RTX; OV: 10 μl of pLVX-IRES-ZsGreen1-mir-223-3p vectors was injected into the lumbar vertebrae between L5 and L6 at TU/ml once a week; IV: 10 μl of pSICOR-shmiR-223-3p vectors was injected into the lumbar vertebrae between L5 and L6 at TU/ml once a week; EV: 10 μl of empty pLVX-IRES-ZsGreen1 vectors was injected into the lumbar vertebrae between L5 and L6 at TU/ml once a week; and EA: the GB30 and GB34 acupoints were stimulated with EA at 2 Hz for 30 min. Sham EA: performs in the same way as EA but without electricity. EA and miR-223-3p were applied every other day starting from seven days after RTX injection.