Bombyxin II Regulates Glucose Absorption and Glycogen Synthesis through the PI3K Signaling Pathway in HepG2 Cells

Figure 2

Expression of BbxII and MBbxII gene in 293FT cells. (a) The gel electrophore result of BbxII gene mRNA expression in 293FT cells by RT-PCR. Line 1: DNA marker 1000, line 2: pcDNA3.1-BbxII/His group, line 3: pcDNA3.1(+) group, and line 4: 293FT cells without gene transfection. (b) The gel electrophore result of MBbxII gene mRNA expression in 293FT cells by RT-PCR. Line 1: 293FT cells without gene transfection, line 2: pcDNA3.1(+)group, line 3: DNA marker 2000, and line 4: pcDNA3.1-MBbxII/His group. (c) Relative quantitative analysis of the expression of BbxII mRNA by real-time quantitative PCR. (d) Relative quantitative analysis of the expression of MBbxII mRNA by real-time quantitative PCR. (e) The protein expression of BbxII in 293FT cells and supernatant observed by Western blot. Line 1: 293FT cells without gene transfection group, line 2: pcDNA3.1(+) group, and line 3: pcDNA3.1-BbxII/His group. (A) Intracellular GAPDH protein. (B) Intracellular BbxII protein. (C) BbxII protein in cell culture supernatant. (f) The protein expression of MBbxII in 293FT cells and supernatant observed by Western blot. Line 1: 293FT cells without gene transfection group, line 2: pcDNA3.1(+) group, and line 3: pcDNA3.1-MBbxII/His group. (A) Intracellular GAPDH protein. (B) Intracellular BbxII protein. (C) MBbxII protein in cell culture supernatant.

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