circRNA circFAT1(e2) Elevates the Development of Non-Small-Cell Lung Cancer by Regulating miR-30e-5p and USP22

<div>USP22 served as the functional protein of circFAT1(e2)/miR-30e-5p. (a) The relative fluorescence intensity was confirmed by the luciferase reporter assay when the cotransfection between USP22 3<span class="nowrap"><svg height="11.4859pt" id="M10" style="vertical-align:-0.04979992pt" version="1.1" viewbox="-0.0498162 -11.4361 3.89517 11.4859" width="3.89517pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.0091,0,0,-0.0091,0,-5.741)"><path d="M310 541L304 571C290 586 211 619 185 610L80 76L131 52L310 541Z"></path></g></svg>-</span>UTR wild-type or mutant and miR-30e-5p or control happened. (b) There was difference between the expression of USP22 in normal tissues and that in NSCLC tissues. (c) After overexpression of miR-30e-5p, USP22 mRNA levels decreased. (d) USP22 mRNA level decreased after knockdown of circFAT1(e2) in A549 and H1299 cells. (e) USP22 mRNA expression can be increased by the miR-30e-5p inhibitor which can also resist the reduction of USP22 mRNA caused by circFAT1(e2) knockdown. <span class="nowrap"><svg height="10.7395pt" id="M11" style="vertical-align:-0.6370001pt" version="1.1" viewbox="-0.0498162 -10.1025 50.9184 10.7395" width="50.9184pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.0091,0,0,-0.0091,0,-5.741)"><path d="M486 158C486 177 478 202 466 220C413 228 386 236 336 262C386 288 413 297 466 304C478 323 486 347 485 366C470 376 444 381 422 380C389 338 368 319 321 288C323 345 329 372 349 422C339 442 322 461 305 470C289 461 271 442 262 422C281 372 287 345 290 288C243 319 222 338 189 380C167 381 142 376 125 366C125 347 133 322 145 304C198 296 225 288 275 262C225 236 198 227 145 220C133 201 125 177 126 158C141 148 167 143 189 144C222 186 243 205 290 236C288 179 282 152 262 102C272 82 289 63 306 54C322 63 340 82 350 102C330 152 324 179 321 236C368 205 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BioMed Research International

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Figure 4

Figure 4: circRNA circFAT1(e2) Elevates the Development of Non-Small-Cell Lung Cancer by Regulating miR-30e-5p and USP22 