Computer-Aid Directed Evolution of GPPS and PS Enzymes

Figure 1

(a) Protein sequence of GPPS. The truncated part of residues 2-80 is shown in red. (b) Protein sequence of PS. Three parts truncated either alone or combined are shown in purple (2-38), blue (39-63), and green (64-80). (c, d) Predicted secondary structures of GPPS (c) and PS (d). (e) Diagram of variants of the GPPS-PS-truncated fusion protein.

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(b)

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