Research Article
Computer-Aid Directed Evolution of GPPS and PS Enzymes
Figure 3
Expression of GPPS/PS variants in E. coli. (a–d) The expression of truncated variants of GPPS/PS fusion proteins in E. coli. Lanes labeled with the symbol “+” denote induction by IPTG. (e) The expression of GPPS/PS fusion proteins with GPPS point mutations under induction of IPTG. Ctrl denotes the “wild-type” GPPS/PS fusion protein without IPTG induction.
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