Computer-Aid Directed Evolution of GPPS and PS Enzymes

Table 2

The name and vector information of each strain and what mutation it denotes.

Strain	Vector	Mutation site
E.GΔ80	pET24a-GPPStra-PS	GPPSΔ2-80
E.PΔ38	pET24a-GPPS-PStra38	PSΔ2-38
E.PΔ63	pET24a-GPPS-PStra63	PSΔ2-63
E.PΔ80	pET24a-GPPS-PStra80	PSΔ2-80
E.GPΔ38	pET24a-GPPStra-PStra38	GPPSΔ2-80 PSΔ2-38
E.GPΔ63	pET24a-GPPStra-PStra63	GPPSΔ2-80 PSΔ2-63
E.GPΔ80	pET24a-GPPStra-PStra80	GPPSΔ2-80 PSΔ2-80
E.G167R	pET24a-GPPS167R-PS	H167R
E.G273R	pET24a-GPPS273R-PS	T273R
E.G138K	pET24a-GPPS138K-PS	R138K
E.G171F	pET24a-GPPS171F-PS	L171F
E.G252F	pET24a-GPPS252F-PS	I252F