Attenuated lncRNA NKILA Enhances the Secretory Function of Airway Epithelial Cells Stimulated by Mycoplasma pneumoniae via NF-κB

Figure 3

NKILA knockdown promoted IκBα phosphorylation and p65 translocation in epithelial cells. (a, b) Total p65 protein level in NKILA-depleted and control A549 and BEAS-2B cells before and after MP infection was measured by western blot and normalized to GAPDH. (c, d) Nuclear p65 phosphorylation level was measured by western blot and histone H3 was the loading control. (e, f) Cytoplasmic IκBα and IκBα phosphorylation levels in NKILA-depleted and control A549 and BEAS-2B cells with or without MP infection were measured by western blot and normalized to β-actin. The samples were derived from the same experiment and gels/blots were processed in parallel. Data are shown as the from three independent experiments (, , and ).

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