Research Article

Attenuated lncRNA NKILA Enhances the Secretory Function of Airway Epithelial Cells Stimulated by Mycoplasma pneumoniae via NF-κB

Figure 4

NKILA inhibits NF-κB activity by binding to IκBα. (a, b) NF-κB activity of A549 and BEAS-2B cells was detected using a dual-luciferase reporter assay. (c) Interaction between NKILA and p65/IκBα revealed by RIP experiments. Cell extracts of MP-stimulated A549 cells were immunoprecipitated with control IgG or anti-p65/IκBα antibody, and the complexes were analyzed for the presence of β-actin mRNA and NKILA by qPCR. Data are shown as the from three independent experiments (, , and ).
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