MicroRNA-27a Promotes Oxidative-Induced RPE Cell Death through Targeting FOXO1

Figure 2

FOXO1 and miRNA-27a expression after blue LED exposure in vivo. (a) The FOXO1 protein levels were examined by immunohistochemistry after blue LED exposure at the indicated times ( μM). (b) The western blot analysis and the statistical results of FOXO1 protein levels in the RPE-choroid-sclera complex at the indicated times after blue LED exposure. (c, d) The FOXO1 mRNA levels and miRNA-27a expression levels of the RPE-choroid-sclera complex were detected by qRT-PCR at the indicated times after blue LED exposure; 2(-ΔΔCT) method was used for quantification analysis. The data are expressed as . , , , and , comparisons versus control, (eye samples in each group).

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