MicroRNA-27a Promotes Oxidative-Induced RPE Cell Death through Targeting FOXO1

Figure 3

The morphological and functional changes after modulating miR-27a-FOXO1 axis in the RD rat model. (a) The relative expression level of miR-27a of rats treated with miR-27a OE (miR-27a overexpression) and miR-27a shRNA. (b) The relative expression level of FOXO1 mRNA of rats treated with FOXO1 OE (FOXO1 overexpression) and FOXO1 shRNA. (c, d) The representative images and mean amplitudes of ERG responses in 3000-lux blue LED-exposed retinas of the control blank and experimental Lentivirus-mediated injection groups at different time points (e, f) H&E staining of retinal sections and the ONL thickness measurement of the control blank Lentivirus and experimental Lentivirus-mediated injection groups at different time points after blue LED exposure ( μM). (c) TUNEL of representative sections from the control blank Lentivirus and experimental Lentivirus-mediated injection groups at different time points (Scale bar =50 μM). The data are analyzed as . , , and , comparisons versus control, (eye samples in each group).

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