MicroRNA-27a Promotes Oxidative-Induced RPE Cell Death through Targeting FOXO1

Figure 4

Establishment of the oxidative stress response model in vitro in ARPE-19 cells. (a) ARPE-19 cells were treated with 100-800 μM H₂O₂ for 0.5 h, and cell viability was measured at a wavelength of by CCK-8 assay. (b, c) ARPE-19 cells were under 100 and 200 μM H₂O₂ treatment for 0.5–3 h, and cell viability was measured by CCK-8 assay. (d, e) Quantitative real-time PCR results of the relative miRNA-27a and FOXO1 mRNA expression level of ARPE-19 cells under H₂O₂ treatment; 2(-ΔΔCT) method was used for quantification analysis. (f) Quantitative real-time PCR was applied to analyze the FOXO1 mRNA expression in ARPE-19 cells transfected with the si-FOXO1 compared with the NC group; 2(-ΔΔCT) method was used for quantification analysis. The data are expressed as . and . All experiments were repeated three times independently.

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