MicroRNA-27a Promotes Oxidative-Induced RPE Cell Death through Targeting FOXO1

Figure 5

miRNA-27a regulates FOXO1 expression through binding to the 3-UTR region. (a) ARPE-19 cells were transfected with negative control, miRNA-27a mimic, and miRNA-27a inhibitor for 48 hrs, and qRT-PCR was applied to show the relative abundance of miRNA-27a in ARPE-19 cells. U6 was applied as an internal control. (b) Negative control, miRNA-27a mimic, and miRNA-27a inhibitor were transfected into ARPE-19 cells for 48 hrs, and the FOXO1 protein levels were measured by western blotting. Actin was used as an internal control. (c) The dual luciferase assay verified the binding site of miRNA-27a to the FOXO1 3-UTR. ARPE-19 cells were transfected with wild-type FOXO1 or mutant-type FOXO1 with negative control and miRNA-27a mimic, respectively, for 48 hrs, and the dual luciferase activity was measured. (d) The 3-untranslated region of FOXO1 mRNA targeted by miRNA-27a was predicated through the TargetScan website. The data are expressed as . and . All experiments were repeated three times independently.

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