MicroRNA-27a Promotes Oxidative-Induced RPE Cell Death through Targeting FOXO1

Figure 6

The miRNA-27a-FOXO1 axis regulates ARPE-19 cell autophagic activity. (a) ARPE-19 cells were treated with different concentrations of H₂O₂, 3-MA, or rapamycin, and the expression of autophagic protein LC3B-II was detected by western blotting. Actin was used as an internal control. (b) Cells were pretreated with vehicle, rapamycin (200 nM), or 3-MA (1 mM), respectively, and then challenged with H₂O₂ (100 μM). LC3B-II protein levels were detected by western blot assay. (c) Western blot results of ARPE-19 cells transfected with miR-27a, miR-27a inhibitor, or miR-NC. (d) ARPE-19 cells were transfected with si-NC or FOXO1 siRNA for 48 hrs, and the expression levels of FOXO1, Atg12, and LC3B-II were examined by western blotting. Actin was used as an internal control. The data are shown as . , , , and . All experiments were repeated three times independently.

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