MicroRNA-27a Promotes Oxidative-Induced RPE Cell Death through Targeting FOXO1

Figure 7

The perturbation of miRNA-27a-FOXO1 axis reduces antioxidative ability of ARPE-19 cell in an autophagy-dependent way. (a) ARPE-19 cells were pretreated with the indicated concentrations of rapamycin and 3-MA for 2 hrs and were stimulated with H₂O₂ (100 μM), and then, the cell viability was measured by CCK-8 assay. (b) ARPE-19 cells were transfected with the negative control, miRNA-27a, and FOXO1 siRNAs for 48 hrs and then were pretreated with the indicated concentrations of rapamycin for 2 hrs. Thereafter, the cells were stimulated with H₂O₂ (100 μM) and the cell viability was measured by CCK-8 assay at 450 nm wavelength. (c) H2DCFDA staining of oxidative APRE-19 cells in the indicated groups ( μM). (d) The fluorescence of intracellular ROS in the indicated groups was measured in a time-dependent manner (starting  min;  min;  μM). Intracellular ROS of ARPE-19 cells stimulated with 100 μM H₂O₂,  nM RAPA, and  mM 3-MA were subjected to flow cytometry analysis. (f) ARPE-19 cells were transfected with the negative control, miRNA-27a, and FOXO1 siRNA, and the intracellular ROS was analyzed by flow cytometry. Rapa: rapamycin. The data are shown as . , , and . The experiments were repeated three times independently.

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