Quantitative Phosphoproteomic Comparison of Lens Proteins in Highly Myopic Cataract and Age-Related Cataract

Table 3

Phosphosites differentially expressed in HMC-C vs ARC-C.

Protein names	Gene name	Accession	Score	Modified sequence	Position	FC	value
Phosphosites exclusively detected in HMC-C
Fructose-bisphosphate aldolase	ALDOA	J3KPS3	80.229	_CQY(ph)VTEK_	208	NaN	≤0.001
N-Acetylserotonin O-methyltransferase-like protein	ASMT	O95671	80.979	_VVLASAS(ph)PR_	21	NaN	≤0.001
Alpha-crystallin A chain	CRYAA	P02489	193.97	_YRLPSNVDQS(ph)ALSCSLSADGMLTFCGPK_	127	NaN	0.047
Beta-crystallin B1	CRYBB1	P53674	163.34	_WNTWS(ph)SSYR_	128	NaN	0.014
Beta-crystallin B3	CRYBB3	P26998	69.122	_CELS(ph)AECPSLTDSLLEK_	42	NaN	≤0.001
Gamma-crystallin C	CRYGC	P07315	111.12	_SCCLIPQT(ph)VSHR_	85	NaN	0.003
Glutathione reductase, mitochondrial	GSR	P00390	74.12	_GHAAFT(ph)SDPKPTIEVSGK_	177	NaN	0.008
Phakinin	BFSP2	Q13515	116.43	_S(ph)SSS(ph)LESPPASR_	35	NaN	≤0.001
Galectin-1	LGALS1	P09382	74.12	_SFVLNLGKDS(ph)NNLCLHFNPR_	39	NaN	≤0.001
Mpv17-like protein 2	MPV17L2	Q567V2	106.13	_LLS(ph)AGQLLFQGR_	14	NaN	0.004
Ubiquitin C variant (fragment)	UBC	Q59EM9	122.44	_TIT(ph)LEVEPSDTIENVK_	30	NaN	≤0.001
14-3-3 protein zeta/delta (fragment)	YWHAB	E7EX29	55.885	_DICNDVLS(ph)LLEK_	99	NaN	0.003
Phosphosites exclusively detected in ARC-C
Alcohol dehydrogenase 1A	ADH1A	V9HW89	124.19	_AMGAAQVVVTDLSATRLS(ph)K_	211	NaN	0.048
Alcohol dehydrogenase 2A	ADH1A	V9HW89	82.885	_EIGADLVLQISKES(ph)PQEIAR_	228	NaN	≤0.001
Carbonyl reductase (NADPH) 1	CBR1	P16152	75.462	_LFS(ph)GDVVLTAR_	30	NaN	≤0.001
Alpha-crystallin B chain	CRYAA	P02511	152.88	_IPADVDPLTITS(ph)SLSSDGVLTVNGPR_	135	NaN	≤0.001
Beta-crystallin B1	CRYBB1	P53674	162.38	_WNTWSS(ph)SYR_	129	NaN	0.016
Protein 4.1	EPB41	P11171	99.021	_QAS(ph)ALIDRPAPHFER_	521	NaN	≤0.001
Glucose-6-phosphate isomerase (fragment)	GPI	A0A0A0MTS2	65.716	_ELQAAGKS(ph)PEDLER_	470	NaN	≤0.001
Heat shock 70 kDa protein 4	HSPA4	P34932	63.709	_AFS(ph)DPFVEAEK_	76	NaN	≤0.001
Phakinin	BFSP2	Q13515	151.44	_S(ph)S(ph)SSLESPPASR_	32	NaN	≤0.001
Phakinin	BFSP2	Q13515	58.693	_VHALEQVSQELET(ph)QLR_	134	NaN	0.006
L-Lactate dehydrogenase A chain	LDHC	P00338	90.614	_S(ph)ADTLWGIQK_	319	NaN	0.028
Phosphoglycerate mutase 1	PGAM1	P18669	56.72	_FSGWYDADLS(ph)PAGHEEAKR_	31	NaN	0.016
Synaptobrevin homolog YKT6	YKT6	O15498	53.033	_IDWPVGS(ph)PATIHYPALDGHLSR_	114	NaN	0.010
14-3-3 protein eta	YWHAH	Q04917	51.147	_KNS(ph)VVEASEAAYK_	145	NaN	≤0.001
Phosphosites with upregulation in HMC-C (selected)
Gamma-crystallin D	CRYGD	P07320	130.01	_HYECSSDHPNLQPY(ph)LSR_	29	29.25	0.006
Alpha-crystallin B chain	CRYAA	P02511	184.11	_RPFFPFHS(ph)PSR_	19	11.99	0.003
Alpha-crystallin A chain	CRYAA	P02489	155.17	_T(ph)LGPFYPSR_	13	5.08	≤0.001
Quinone oxidoreductase PIG3	TP53I3	Q53FA7	141.54	_RGS(ph)LITSLLR_	260	3.92	≤0.001
Alpha-crystallin A chain	CRYAA	P02489	123.19	_QS(ph)LFRTVLDSGISEVR_	51	2.77	0.013
Phosphosites with downregulation in HMC-C (selected)
Phakinin	BFSP2	Q13515	174.62	_SS(ph)SSLES(ph)PPASR_	38	0.05	0.048
Filensin	BFSP1	Q12934	173.55	_VRS(ph)PKEPETPTELYTK_	454	0.20	0.026
Filensin	BFSP1	Q12934	173.55	_VRS(ph)PKEPET(ph)PTELYTK_	460	0.26	≤0.001
Retinal dehydrogenase 1	ALDH1A1	P00352	78.921	_YILGNPLT(ph)PGVTQGPQIDKEQYDK_	337	0.28	≤0.001
Brain acid soluble protein 1	BASP1	P80723	127.95	_AEGAATEEEGT(ph)PK_	36	0.30	0.008