Research Article
lncRNA MALAT1 Regulates Mouse Granulosa Cell Apoptosis and 17β-Estradiol Synthesis via Regulating miR-205/CREB1 Axis
Figure 1
Knockdown of MALAT1 affects mGC proliferation and apoptosis. (a) The expression of MALAT1 in HFs, EAFs, and PAFs was determined using qRT-PCR. EAFs: early atretic follicles; HFs: healthy follicles; PAFs: progressively atretic follicles. (b) The expression of MALAT1 was examined in mGCs transfected with si-MALAT1 or si-NC. (c–e) Cell apoptosis and caspase-3/9 activities were determined in mGCs transfected with si-MALAT1 or si-NC. Data are the () of one representative experiment, , vs. the HFs or si-NC group. mGCs: mouse granulosa cells; MALAT1: metastasis-associated lung adenocarcinoma transcript 1; qRT-PCR: quantitative RT-PCR.
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