lncRNA MALAT1 Regulates Mouse Granulosa Cell Apoptosis and 17β-Estradiol Synthesis via Regulating miR-205/CREB1 Axis

Figure 4

MALAT1 exerts a biological role in mGCs by regulating the miR-205/CREB1 axis. (a, b) The expression of CREB1 on mRNA and protein levels was examined in mGCs transfected with si-NC, si-MALAT, and si-MALAT1+anti-miR-205. (c–g) The apoptosis, caspase-3/9 activities, and E2 and P4 production were determined in mGCs transfected with si-NC, si-MALAT, si-MALAT1+anti-miR-205, and si-MALAT1+CREB1. Data are the () of one representative experiment, , vs. the si-NC group. CREB1: encoding cyclic AMP response element- (CRE-) binding protein 1; mGCs: mouse granulosa cells; MALAT1: metastasis-associated lung adenocarcinoma transcript 1.

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