Development of a Sandwich Enzyme-Linked Immunosorbent Assay for Detection and Quantification of Clam Residues in Food Products

Figure 1

Electrophoretic pattern of clam protein extracts and immunoreactivity of clam antisera. (a) SDS-PAGE with Coomassie staining. (b) Immunoblot with sheep serum. (c) Immunoblot with rabbit serum. Mix (Ret.): mix of Atlantic Surf clam and ocean quahog calm, retorted; Surf: Atlantic Surf clam; Quahog: quahog clam; MWs: molecular weight markers (indicated in kDa at right margin of (a)); +: cooked material; -: raw material. (b) and (c) are aligned with (a) to allow estimating the MWs on the immunoblot, as MW markers do not stain in immunoblot.

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(b)

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