Research Article

Development of a Sandwich Enzyme-Linked Immunosorbent Assay for Detection and Quantification of Clam Residues in Food Products

Table 3

Recovery of spikes of clam protein in different model food products.

Spike level (ppm clam protein)Tomato juicePotato cream soupRetorted potato cream soup
Sample A (ppm)Sample B (ppm)Mean (ppm)Recovery (%)Sample A (ppm)Sample B (ppm)Mean (ppm)Recovery (%)Sample A (ppm)Sample B (ppm)Mean (ppm)Recovery (%)

0<<<N.A.<<<N.A.<<<N.A.
5<<<N.A.<<<N.A.<<<N.A.
108.06.87.47411.211.411.3113<<<N.A.
2515.517.016.36524.025.524.899<<<N.A.
5033.833.633.76745.543.144.38911.612.512.124
10070.366.568.46882.565.073.87429.526.327.928

< Below LLOQ, 6 ppm; N.A.: not applicable. All standards were extracted in duplicate, and each extract was analyzed in triplicate in two different ELISA trials.