Human Umbilical Cord Mesenchymal Stem Cells Ameliorate Hepatic Stellate Cell Activation and Liver Fibrosis by Upregulating MicroRNA-455-3p through Suppression of p21-Activated Kinase-2

<div>HUC-MSCs inhibit proliferation and activation of HSCs. (a) CCK8 assay analysis of HUC-MSCs on inhibition of TGF<i>β</i>1-mediated proliferation in LX-2 cells. (b) The mRNA levels of <i>α</i>-SMA and Col1<i>α</i>1 were measured by qRT-PCR. (c) The protein levels of <i>α</i>-SMA and Col1<i>α</i>1 were measured by Western blot. (d) Quantitation of <i>α</i>-SMA and Col1<i>α</i>1 levels from three independent Western blot analyses. (e) Immunofluorescence images of LX-2 cells, the red fluorescence represents <i>α</i>-SMA, and the green fluorescence represents Col1<i>α</i>1. Scale bars, 200 <i>μ</i>m. (f) Quantification of <i>α</i>-SMA and Col1<i>α</i>1 positive areas. Percentages of immunoreactive areas were expressed as relative values to those in the LX-2 group. 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BioMed Research International

fig1

Figure 1

Figure 1: Human Umbilical Cord Mesenchymal Stem Cells Ameliorate Hepatic Stellate Cell Activation and Liver Fibrosis by Upregulating MicroRNA-455-3p through Suppression of p21-Activated Kinase-2 

Figure 1 | Human Umbilical Cord Mesenchymal Stem Cells Ameliorate Hepatic Stellate Cell Activation and Liver Fibrosis by Upregulating MicroRNA-455-3p through Suppression of p21-Activated Kinase-2 