Human Umbilical Cord Mesenchymal Stem Cells Ameliorate Hepatic Stellate Cell Activation and Liver Fibrosis by Upregulating MicroRNA-455-3p through Suppression of p21-Activated Kinase-2

Figure 2

HUC-MSCs promote inactivation of LX-2 cells by upregulating miR-455-3p. (a) Venn diagram of DEMs about liver fibrosis from two miRNA profile data. (b) Comparison of qRT-PCR results of five selected DEMs in LX-2 cells treated with or without TGFβ1. (c) Comparison of the expression levels of five selected DEMs in TGFβ1-activated LX-2 cells cultured alone or cocultured with HUC-MSCs. (d) The expression levels of miR-455-3p were examined in TGFβ1-treated LX-2 cells cocultured with HUC-MSCs and then were transfected with miR-455-3p mimics, mimics NC, inhibitors, or inhibitors NC, respectively. (e) The protein levels of α-SMA measured by Western blot in each group. (f) Quantitation of α-SMA levels from three independent Western blot analyses. The quantitative data are represented as the . Each experiment was repeated three times. and .

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