Human Umbilical Cord Mesenchymal Stem Cells Ameliorate Hepatic Stellate Cell Activation and Liver Fibrosis by Upregulating MicroRNA-455-3p through Suppression of p21-Activated Kinase-2

Figure 3

miR-455-3p regulates PAK2 expression by directly targeting the 3 UTR of its mRNA. (a) Predicted miR-455-3p targeting sequence in 3 UTR of WT or MUT PAK2 mRNA. (b) Dual-luciferase reporter assay of LX-2 cells cotransfected with WT or MUT PAK2 3' UTR reporter and miR-455-3p mimics or mimics NC. (c) PAK2 protein expression analysis in LX-2 cells transfected with miR-455-3p mimics using Western blotting. (d) Quantitation of PAK2 protein levels from three independent Western blot analyses. The quantitative data are represented as the . Each experiment was repeated three times. and .

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