In Silico Analysis of High-Risk Missense Variants in Human ACE2 Gene and Susceptibility to SARS-CoV-2 Infection

Table 3

Prediction of effect of nsSNPs on ACE2 protein stability using MUpro.

			Method 1: SVM (support vector machine)		Method 2: neural network
Mutation	Delta G	Prediction	Confidence score	Effect	Confidence score	Effect
R219C	-0.89	Decrease	-0.082	Decrease	-0.57	Decrease
R219H	-1.16	Decrease	-0.089	Decrease	-0.51	Decrease
M383T	-1.56	Decrease	-0.94	Decrease	-0.70	Decrease
P389H	-0.45	Decrease	-0.55	Decrease	-0.85	Decrease
D427Y	-0.79	Decrease	0.16	Increase	-0.67	Decrease
R514G	-1.15	Decrease	-0.76	Decrease	-0.98	Decrease
R708W	-0.99	Decrease	-0.88	Decrease	-0.98	Decrease
R710C	-1.17	Decrease	-0.83	Decrease	-0.87	Decrease
R710H	-1.66	Decrease	-0.07	Decrease	-0.98	Decrease
R716C	-1.34	Decrease	-0.10	Decrease	-0.82	Decrease
L731F	-0.87	Decrease	-0.04	Decrease	-0.66	Decrease
R768W	-1.89	Decrease	-1	Decrease	-0.99	Decrease

Delta G: energy change value; Confidence score: (>0: increase the stability, <0: decrease the stability).