In Silico Analysis of High-Risk Missense Variants in Human ACE2 Gene and Susceptibility to SARS-CoV-2 Infection

Table 5

Cumulative score calculation of tested nsSNPs.

Mutation	SIFT	PolyPhen	PROVEAN	PANTHER	I.Mutant	MUpro	ModPred	Cumulative score
R219C	1	1	0	1	0	1	1	5
R219H	1	1	0	1	1	1	1	6
M383T	1	1	1	1	0	1	0	5
P389H	1	1	1	1	0	1	0	5
D427Y	1	1	1	0	1	1	1	6
R514G	1	1	1	1	1	1	0	6
R708W	1	1	1	1	1	1	1	7
R710C	1	1	1	1	1	1	0	6
R710H	1	1	0	1	1	1	0	5
R716C	1	1	0	1	1	1	1	6
L731F	1	1	0	1	0	1	0	4
R768W	1	1	1	1	1	1	0	6

The highly pathogenic nsSNPs by all the seven tools, the highly pathogenic nsSNPs by at least 6 tools, 0 indicates neutral prediction, 1 indicates deleterious prediction.