Ding Chuan Tang Attenuates Airway Inflammation and Eosinophil Infiltration in Ovalbumin-Sensitized Asthmatic Mice

Figure 1

Experimental procedure for OVA sensitization and Ding Chuan Tang treatment. Eight-week-old mice were sensitized by intraperitoneal (I.P.) injection of 50 μg ovalbumin (OVA) mixed with 0.8 mg of aluminum hydroxide (Al(OH)₃) gel in a total volume of 200 μl on days 1, 2, 3, and 14. The mice were then challenged by inhalation (I.H.) of 2% aerosolized OVA on days 14, 17, 21, 24, and 28. OVA was replaced by normal saline (N.S.) in the normal control group. DCT (500 mg/kg) in 250 μl was given orally from day 14 to 28. N.S. was given to the normal control group and OVA-challenged group for comparison. Airway hyperresponsiveness (AHR) was determined on day 29, and mice were sacrificed 24 hours later. I.P.: intraperitoneal; I.H.: inhalation; N.S.: normal saline; DCT: Ding Chuan Tang.