RBM23 Drives Hepatocellular Carcinoma by Activating NF-κB Signaling Pathway

Figure 3

RBM23 activates NF-κB activity and promot05es the expression of its target proangiogenesis genes. (a) Alteration of RBM23 did not affect the expression of the key molecules of related signaling pathways. (b) RBM23 positively regulated RelA/p65 without influencing the expression of other molecules in the NF-kappaB signaling pathway. (c) Immunofluorescence results showed that overexpression of RBM23 can increase the expression of RelA/p65 in Huh-7 cells (1 cm represents 100 μm). (d) Western blot analysis of nuclear p65 and cytoplasmic p65 in different cells. (e) ELISA analysis of the IL-8 content from different supernatant of cells with different treatments.

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