[Retracted] Pentoxifylline Can Reduce the Inflammation Caused by LPS after Inhibiting Autophagy in RAW264.7 Macrophage Cells

Figure 5

(a) IL-1β and (b) TNF-α level in supernatants of RAW264.7 cells treated with PTX with or without autophagy inhibitor 3-MA/LY294002 was measured by ELISA. The cells were pretreated with 0.5 mg/mL PTX with or without autophagy inhibitor 3-MA and LY294002 for 12 h and then treated with 100 ng/mL LPS and 20 ng/mL IFN-γ for 12 h. Finally, the supernatants were collected for ELISA measurement (; ^#, ^###; ^$$). (c) TNF-α and NLRP3 protein level in RAW264.7 cells cultured with or without the autophagy inhibitor 3-MA/LY294002 detected by Western blotting, and β-actin was the internal control. The cells were firstly treated with 0.5 mg/mL PTX for 12 h and then treated with 100 ng/mL LPS and 20 ng/mL IFN-γ for 12 h. The quantitative analysis of different protein expressions is shown by a bar graph (, ; ^#, ^###; ^$, ^$$).

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