[Retracted] Pentoxifylline Can Reduce the Inflammation Caused by LPS after Inhibiting Autophagy in RAW264.7 Macrophage Cells

Figure 6

(a) AMPK and p-AMPK protein level in RAW264.7 cells detected by Western blotting. The cells were initially treated with 0.5 mg/mL PTX for 12 h and then treated with 100 ng/mL LPS and 20 ng/mL IFN-γ for 6 h (; ^###). (b, c) LC3 and Beclin 1 protein level in RAW264.7 cells cultured with or without the activator A-769662 of AMPK signaling pathways detected by Western blotting, and β-actin expression was the internal control. The cells were pretreated with PTX (0.5 mg/mL) for 12 h. The quantitative analysis of different protein expressions is shown by a bar graph (, ^#, ^##, ^###). (d) TNF-α and NLRP3 protein level in RAW264.7 cells with or without activator A-769662 of AMPK signaling pathways was examined by Western blotting; and β-actin expression was the internal control. The cells were initially treated with 0.5 mg/mL PTX for 12 h and then treated with 100 ng/mL LPS and 20 ng/mL IFN-γ for 6 h. The quantitative analysis of different protein expressions is shown by a bar graph (; ^##; ^##, ^###).

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