Renal Protective Effect of Beluga Lentil Pretreatment for Ischemia-Reperfusion Injury

Figure 5

Effects of beluga lentil pretreatment on inflammatory responses after ischemia/reperfusion injury. (a) Immunohistochemical analysis examining F4/80 and CD4 to detect immune cell infiltration. (b) Detection of proinflammatory cytokines IL-1β, TNF-α, and IL-6 mRNA expression. (c) Detection of anti-inflammatory cytokines TGF-β, IL-10, and IL-22 mRNA expression. Normal: control group; untreated: saline-treated group; low: 2 mg beluga lentil pretreatment group; high: 8 mg beluga lentil pretreatment group. Beluga lentil was orally administered for 14 days, and then ischemia was performed for 20 min and reperfusion was performed for 30 min. F4/80: macrophage marker; CD4: cluster of differentiation 4; IL-1β: interleukin 1 beta; IL-6: interleukin 6; TNF-α: tumor necrosis factor-alpha; IL-10: interleukin 10; TGF-β1: transforming growth factor beta 1.

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