[Retracted] Protein Arginine Methyltransferase 5 Promotes the Migration of AML Cells by Regulating the Expression of Leukocyte Immunoglobulin-Like Receptor B4

Figure 2

Detection of expression in human samples and AML cell lines. (a) Western blot was used to detect the protein level expression in PRMT5 (normal, THP-1, MV-4-11, kasum-1, and HL-60), respectively. β- Actin was used as the internal control. (b) Western blot and subsequent grayscale analysis revealed the expression of PRMT5. Downregulation of PRMT5 gene by siRNA could inhibit the migration, invasion, and adhesion of MV-4-11. (c) PRMT5 silencing by siRNA on AML. After treatment with si-PRMT5 or NC siRNA for 48 h, the expression of PRMT5 in protein (upper) and mRNA (lower) level was significantly decreased in MV-4-11 cells. (d) Adhesion assay exhibited that the adhesion capability of MV-4-11 cells in the si-PRMT5 group was significantly lower than that in the wild type group and si-CN group. (e) Transwell assay was performed to detect the migration of MV-4-11 cells in the wild type group, si-CN group, and si-PRMT5 group. (f) Quantitative analysis of the number of migrating cells in each group after 24 h indicated that the number of migrating cells in the si-PRMT5 group was significantly decreased. (g) After downregulation of PRMT5 by siRNA, the invasion of MV-4-11 cells in each group was observed. (h) Quantitative analysis revealed that the number of invasion in MV-4-11 cells was significantly decreased. Data are expressed by (; ; ). The 2-Δ CT method was used for quantitative presentation of relative mRNA levels. The error bar represents ±SD. (). The images of (c, e) are under 100x magnification.

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