[Retracted] Protein Arginine Methyltransferase 5 Promotes the Migration of AML Cells by Regulating the Expression of Leukocyte Immunoglobulin-Like Receptor B4

Figure 3

Downregulation of PRMT5 gene by siRNA can inhibit the migration, invasion, and adhesion of THP-1. (a) After treatment with si-PRMT5 or NC siRNA for 48 h, the expression of PRMT5 in protein (upper) and mRNA (lower) level was significantly decreased in THP-1 cells. (b) Adhesion assay revealed that the adhesion capability of THP-1 cells in the si-PRMT5 group was significantly decreased than that in the wild type group and si-CN group. (c) Transwell assay was used to detect the migration of THP-1 cells in the CON group, si-CN group, and si-PRMT5 group. (d) Quantitative analysis of the number of migrating cells in each group after 24 h indicated that the number of migrating cells in the si-PRMT5 group was significantly decreased. (e) Invasion assay was performed to detect the migration of THP-1 cells in the CON group, si-CN group, and si-PRMT5 group. (f) Quantitative analysis of the number of invasion cells in each group after 24 h indicated that the number of invasion cells in the si-PRMT5 group was significantly decreased. All the experiments were performed in triplicate. Data are expressed by (; ). 2-Δ CT was used for quantitative presentation of relative mRNA levels. The error bar represents ±SD (). The images of (c, e) are under 100x magnification.

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