[Retracted] Protein Arginine Methyltransferase 5 Promotes the Migration of AML Cells by Regulating the Expression of Leukocyte Immunoglobulin-Like Receptor B4

Figure 4

PRMT5 inhibitor (EPZ015666) attenuates the migration, invasion, and adhesion of AML cells. (a) After EPZ015666 treatment in MV-4-11 cells for 24 h, the numbers of cell migration in the CON group and EPZ015666 group were detected by Transwell migration study. (b) Quantification analysis of (a) indicated that the number of cells in the inhibitor group was significantly less than that in the CON group. (c) After EPZ015666 treatment in MV-4-11 cells for 24 h, the number of cell invasion in the CON group and inhibitor group was detected by Transwell invasion assay. (d) Quantification analysis of (c) indicated that the number of invasion cells in the inhibitor group was significantly less than that in the CON group. (e) After EPZ015666 treatment in MV-4-11 cells for 24 h, the protein expression of PRMT5 was changed. (f) After EPZ015666 treatment in MV-4-11 cells for 24 h, cell adhesion was detected, and the number of adherent cells in the inhibitor group was found to be significantly less than that in the CON group. After EPZ015666 treatment in THP-1 cells for 24 h, the (g, h) migration, (j, k) invasion, and (l) adhesion of THP-1 cells were detected. The above indexes in the inhibitor group were significantly lower than those in the CON group. (i) After EPZ015666 treatment in THP-1 cells for 24 h, the protein level of PRMT5 was changed. All the experiments were performed in triplicate. Data are expressed by (; ). The representative images are under 100x magnification.

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