[Retracted] Protein Arginine Methyltransferase 5 Promotes the Migration of AML Cells by Regulating the Expression of Leukocyte Immunoglobulin-Like Receptor B4

Figure 6

The effect of PRMT5 knockdown by siRNA on several signaling pathways in AML cells. (a) Western blot was used to detect the changes of p/T-AKT, p/T-ERK, p/T-mTOR, p/T-NF-κB, and p/T-PI3K in MV-4-11 cells after siRNA knockdown of PRMT5 gene. (top) Western blot images (β-actin as loading control); (bottom) image grayscale analysis of the corresponding Western blot images in triplicates. The results indicated that the expression levels of p-ERK, p-AKT, and p-mTOR were decreased in the si-PRMT5 group. (b) Western blot was used to determine the changes of p/T-AKT, p/T-ERK, p/T-mTOR, p/T-NF-κB, and p/T-PI3K in THP-1 cells after siRNA knockdown of PRMT5 gene. (top) Western blot images (β-actin as loading control); (bottom) image grayscale analysis of the corresponding Western blot images in triplicates. The results suggested that the expression levels of p-ERK, p-AKT, p-NF-κB, and p-mTOR were decreased in the si-PRMT5 group. All the experiments were repeated by three times. Data are expressed by (; ; ).

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