MicroRNA-103a-3p Promotes Cell Proliferation and Invasion in Non-Small-Cell Lung Cancer Cells through Akt Pathway by Targeting PTEN

Figure 1

miR-103a-3p promotes NSCLC cell proliferation and invasion. (a) TCGA database analysis of miR-103a-3p expression in NSCLC samples and normal lung samples. (b) Relative expression of miR-103a-3p in NSCLC cell lines (A549, NCI-H157, NCI-H1299, and NCI-H1975) and BEAS-2B were detected by qRT-PCR. A549 and NCI-H1299 cells were transfected with miR-103a-3p mimics or inhibitor for 48 h before the following detections. (c) Relative expression of miR-103a-3p was measured by qRT-PCR. (d) Effect of miR-103a-3p on cell proliferation was examined by MTT assay. (e) Effect of miR-103a-3p on colony-forming ability was detected by the colony formation assay. (f) Effect of miR-103a-3p on invasive capability was assessed by Transwell invasion assay (, , and ).

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