Propranolol Suppresses Proliferation and Migration of HUVECs through Regulation of the miR-206/VEGFA Axis

Figure 4

VEGFA is a target gene of miR-206. (a) Predicted binding sequences between miR-206 and VEGFA. (b) The relationship between miR-206 and VEGFA was verified by dual-luciferase reporter assay. , comparing with the agomir control group; ^##, comparing with the propranolol group; . (c) The mRNA level of VEGFA in HUVECs was detected with RT-qPCR after miR-206 antagomir transfection. (d)–(g) The protein levels of VEGFA, p-Akt, Akt, p-ERK, and ERK were determined by western blotting, and the relative expression of proteins was normalized with β-actin. , comparing with the control group; ^##, comparing with the propranolol group; .

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