Association of IL-1β, NLRP3, and COX-2 Gene Polymorphisms with Autoimmune Thyroid Disease Risk and Clinical Features in the Iranian Population

Table 1

Primers and restriction enzymes used for RFLP-PCR method.

Gene and SNP number	Primers	Restriction enzyme	DNA fragment size (bp)
IL-1β (rs1143634)	F: GTTGTCATCAGACTTTGACC; R: TTCAGTTCATATGGACCAGA	Taq I
COX-2 (rs2745557)	F: GAGGTGAGAGTGTCTCAGAT; R: TCTCGGTTAGCGACCAATT	Taq I
NLRP3 (rs3806265)	F: TTGGCAGGTGGACAGCAGCA; R: GACCCCAAACATCCCCCAAATCA	Pvu II

F: forward; R: reverse.