Protective Effect and Mechanism of Bone Morphogenetic Protein-4 on Apoptosis of Human Lens Epithelium Cells under Oxidative Stress

Figure 6

(a) The production of cellular ROS detected by DCF fluorescence. The control group showed minimal ROS production; however, in the H₂O₂ group, the amount of ROS, which is indicated by green fluorescence, significantly increased. The green fluorescence was attenuated in the H₂O₂+BMP-4 group, and the relative fluorescence intensity was significantly reduced compared with that in the H₂O₂ group. (b) Fluorescence intensity quantification of ROS production (green channel). (c) Caspase-3 activity in HLE-B3.

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