Mechanistic Investigation on the Regulation of FABP1 by the IL-6/miR-603 Signaling in the Pathogenesis of Hepatocellular Carcinoma

Figure 4

IL-6 induces the expression of miR-603, which promotes the proliferation and migration of HCC cells. (a) miRNA array after IL-6 treatment in Huh-7 cells. Differentially expressed miRNAs were profiled in three cases of DEPC water induction (CON-1, CON-2, and CON-3) and IL-6 induction (IL6-1, IL6-2, and IL6-3) using miRNA microarrays. A total of 77 deregulated miRNAs in the IL-6 treatment group (). The miR-603 was marked in red square. (b) miR-603 was higher in the IL-6 treatment group than in the control groups (). The top bar represents miRNA expression levels from -2 (green) to +2 (red). The individual identity of significantly deregulated miRNAs is shown on the right. (c) miR-603 expression levels in HCC and adjacent tissues. Data are expressed as the , in each group. . (d) Cell proliferation after miR-603 overexpression. The miR-603 mimic or nonspecific mimic (NSM) was used to transfect cells, while nuclei were stained with DAPI in blue. Proliferative cells were stained with EdU-Apollo567 in red. 10 randomly selected fields were checked under a fluorescence microscope. (e) Quantification of the proliferation rate (%). The proliferation ratio was calculated as the by ImageJ software. Data are expressed as the , in each group. . (f) Huh-7 cell migration showed by the scratch assay after FABP1 overexpression. (g) Quantification of the migration rate (%). 10 randomly selected fields were checked under an inverted microscope. Data are expressed as the , in each group. .

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