[Retracted] Downregulation of microRNA-15b-5p Targeting the Akt3-Mediated GSK-3β/β-Catenin Signaling Pathway Inhibits Cell Apoptosis in Parkinson’s Disease

Figure 6

Suppression of miR-15b-5p inhibited MPTP-induced apoptosis by regulating Akt3 in vivo. (a) Colorimetric assay of caspase-3 activity: downregulating miR-15b-5p and upregulating Akt3 suppressed caspase-3 activity. . (b) Protein expression of Bcl2, Bax, caspase-3, Akt, Gsk3β, and β-catenin in SH-SY5Y cells at 7 d after treatment to verify the role of miR-15b-5p and Akt3. Inhibition of miR-15b-5p and forced Akt3 attenuated expression of proapoptosis proteins. (c) Protein expression of Bcl2, Bax, caspase-3, Akt, Gsk3β, and β-catenin in SH-SY5Y cells at 21 d after treatment to verify the role of miR-15b-5p and Akt3. Inhibition of miR-15b-5p and forced Akt3 attenuated expression of proapoptosis proteins.

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