Suppression of lncRNA MALAT1 Reduces LPS- or IL-17A-Induced Inflammatory Response in Human Middle Ear Epithelial Cells via the NF-κB Signaling Pathway

Figure 3

Knockdown of MALAT1 inactivates the NF-κB pathway. (a) The level of MALAT1 was explored by RT-qPCR assay in control, LPS, or IL-17A group. (b) The knockdown efficacy of sh-MALAT1 in HMEECs was explored by RT-qPCR analysis. (c–h) The effect of MALAT1 on protein levels of cytoplasm p65, nucleus p65, p-IκBα, and IκBα in HMEECs under LPS or IL-17A treatment. (i, j) Immunofluorescence assay was conducted to assess the nuclear translocation of p-NF-κB in HMEECs under LPS or IL-17A treatment. , .

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