Reevaluation of NOD/SCID Mice as NK Cell-Deficient Models

Figure 5

Assay of IFN-γ in cells and cell culture supernatant in vitro. The splenocytes from naïve (a–e) or poly(I:C)-treated (f, g) mice were cultured with cytokines for 48 h. (a) Representative FCM data show IFN-γ in and the expression of DX5 on the splenocytes from CB17/SCID and NOD/SCID mice. (b) Statistical analysis of the FCM data for IFN-γ and DX5 expression. (c) Statistical analysis of the ELISA data for IFN-γ in the culture supernatant. (d) Representative FCM data show the IFN-γ expression gated on DX5⁺ populations of the splenocytes from naïve mice. (e) Statistical analysis of the FCM data for the IFN-γ expression on splenic DX5⁺ populations. (f) Representative FCM data show the IFN-γ expression gated on DX5⁺ populations of the splenocytes from poly(I:C)-treated mice. (g) Statistical analysis of the FCM data for the IFN-γ expression on DX5⁺ populations of the splenocytes from poly(I:C)-treated mice. FCM histograms show representative data of three independent experiments. Results are presented as . mice per group. Values of were considered statistically significant.

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