Elevated Circular RNA PVT1 Promotes Eutopic Endometrial Cell Proliferation and Invasion of Adenomyosis via miR-145/Talin1 Axis

Figure 4

miR-145 rescued the promoting effect of circPVT1 on adenomyotic eutopic endometrial cells. (a, b) The proliferation capacity of Eu_EEC and Eu_ESC was evaluated by Cell-Counting Kit-8 (CCK-8) assays after transfection. (c, d) Colony formation assays were performed to assess the colony formation ability of Eu_EEC and Eu_ESC transfected with GV367-circPVT1, miR-145 mimics, or GV367-circPVT1+miR-145 mimics. (e, f) Different effects of GV367-circPVT1, miR-145 mimics, and GV367-circPVT1+miR-145 mimic treatment on the cell invasion of Eu_EEC and Eu_ESC. The CNC group represented cells without any transfection; GV367-circPVT1, circPVT1 overexpression; miR-145 mimics, miR-145 upregulation; GV367-circPVT1+miR-145 mimics, cells cotransfected with circPVT1 overexpression and miR-145 upregulation vectors. At least triplicate independent experiments were performed. All data were presented as . , , , and ; NS: no significance.

(a)

(b)

(c)

(d)

(e)

(f)