Elevated Circular RNA PVT1 Promotes Eutopic Endometrial Cell Proliferation and Invasion of Adenomyosis via miR-145/Talin1 Axis

Figure 5

Talin1, a downstream target of miR-145, was positively affected by circPVT1. (a) The predicted binding sites between miR-145 and Talin1 through bioinformatics. (b) Wide-type Talin1 (Talin1-WT) or mutant-type Talin1 (Talin1-MT) luciferase reporter was cotransfected into Eu_EEC and Eu_ESC with miR-145 mimics or its negative control (miR-145 NC). The relative luciferase activity was determined. (c, d) The relative mRNA or protein levels of Talin1 in ADS () and normal uterine endometrium () were evaluated by qRT-PCR and immunohistochemical staining, respectively. The magnifications of the micrographs were ×400. Scale bars represented 5 μm. (e, f) The relative mRNA or protein levels of Talin1 in ADS and normal uterine endometrial cells were detected by qRT-PCR and Western blot. (g–j) circPVT1 overexpression or miR-145 knockdown increased the mRNA and protein expression of Talin1 in Eu_EEC and Eu_ESC, whereas cotransfection with miR-145 mimics or sh-circPVT1 got Talin1 expression abolished. ADS_Euc, adenomyotic eutopic endometrium; ADS_Ec, adenomyotic ectopic endometrium; Con_En, the normal uterine endometrium as control; Eu_EEC, adenomyotic eutopic endometrial epithelial cells; Eu_ESC, adenomyotic eutopic endometrial stromal cells; GV367-circPVT1, circPVT1 overexpression; sh-circPVT1, knockdown of circPVT1; miR-145 mimics, miR-145 upregulation; miR-145 inhibitor, inhibition of miR-145. At least triplicate independent experiments were performed. All data were presented as . , , , and ; NS: no significance.

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