Research Article
Acetyl-L-Carnitine Induces Autophagy to Promote Mouse Spermatogonia Cell Recovery after Heat Stress Damage
Figure 4
Effect of ALC on heat stress-induced apoptosis in GC-1 cells. (a) The cells were labeled with AO/EB and then analyzed by fluorescence microscope. (b) Data were expressed as the percent of apoptotic cells obtained from the histogram statistics all quantitative. (c) The mRNA levels of Bcl-2, Bax, and caspase3. (d) Immunoblot analysis of apoptosis-related proteins. (e, f) The protein levels of Bcl-2, Bax, caspase3, and Bcl-2/Bax. The data are presented as the , , , . CON: control group; HS: recover at 6 h; HS-ALC: recover at 6 h with ALCAR.
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