ICT1 Promotes Osteosarcoma Cell Proliferation and Inhibits Apoptosis via STAT3/BCL-2 Pathway

Figure 1

The expression of ICT1 is closely related to the overall survival, and ICT1 promotes proliferation and inhibits apoptosis of OS cells in vitro. (a) The mRNA expression patterns of ICT1 in normal osteoblast cell line (hFOB1.19) and OS cell lines (U-2OS, MNNG-HOS, and 143B) by qPCR. Values are , , (Student’s -test). (b) The expression patterns of ICT1 in normal osteoblast cell line (hFOB1.19) and OS cell lines (U-2OS, MNNG-HOS, and 143B) by western blotting. (c) Kaplan–Meier analysis of overall survival rate related to the expression of ICT1 in 88 OS cases based on a human osteosarcoma gene expression database (https://hgserver1.amc.nl/cgi-bin/r2/main.cgi). (d, e) Knockdown efficacy of ICT1 in wild OS cells (U-2OS and 143B) was determined by qPCR. Values are , (Student’s -test). (f) Knockdown efficacy of ICT1 in wild OS cells (U-2OS and 143B) was determined by western blotting. (g, h) Knockdown of ICT1 inhibited U-2OS and 143B cell proliferation using the cell counting kit (CCK)-8 assay. Values are , (Student’s -test). (i) Knockdown of ICT1 suppressed OS cell (U-2OS and 143B) proliferation using colony formation assay. Values are , (Student’s -test). (j, k) Knockdown of ICT1 significantly induced U-2OS and 143B cell apoptosis. Values are , (Student’s -test).

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