Construction of miRNA-mRNA-TF Regulatory Network for Diagnosis of Gastric Cancer

Figure 4

Construction of miRNA-mRNA-TF regulatory network and validation of RFX6 expression level. (a) Three miRNAs (miR-199a-3p/miR-199b-3p, miR-125b-5p, and miR-199a-5p) and transcription factor (TF) RFX6 coregulate gene expression in the miRNA-mRNA-TF regulatory network, and miRNAs negatively regulate their expression levels by binding to the mRNA 3-UTR region of 17 genes. RFX6 activates transcription by forming a heterodimer with RFX3 and binding to the X-box in the target gene promoter. (b) and (c) Compared with normal gastric tissue, RFX6 was significantly downregulated in GC tissue.

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